Ctll-2 ed50

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August undefined, 2024

WebData Figures. (A) The biological activity of Human Recombinant IL-2, ACF was tested by its ability to promote the proliferation of CTLL-2 cells. The EC50 is defined as the effective concentration of the cytokine at which cell proliferation is at 50% of maximum. The EC50 in the example above is 1.99 ng/mL. (B) 1 μg of Human Recombinant IL-2 ... WebBioactivity: The ED50 of this protein, as determined by increased proliferation of mouse CTLL-2 cells stimulated with 1 ng/mL IL-2, is 0.5-1.5 µg/mL. Endotoxin: Less than 0.1 …

Human IL15RA protein orb594809 from Biorbyt Biocompare.com

WebBiological Activity: The ED50 as determined by its ability to block human IL-15-induced proliferation of CTLL‑2 mouse cytotoxic T cells is less than 10 ng/ml. Sequence ITCPPPMSVE HADIWVKSYS LYSRERYICN SGFKRKAGTS SLTECVLNKA TNVAHWTTPS LKCIRDPALV HQRPAPPSTV TTAGVTPQPE SLSPSGKEPA … WebMouse IL-2, amino acids Phe22-Gln169 (Accession# NM_008366), was expressed in E. coli . The 148 amino acid recombinant protein has a predicted molecular mass of 17.2 kD. The predicted N-terminal amino acid is Phe. >98%, as determined by Coomassie stained SDS-PAGE and HPLC analysis. Lyophilized, carrier-free. how it up sims 4 mod

CTLL-2 - TIB-214 ATCC

WebED50 is < 10 ng/mL as determined by the dose-dependant stimulation of the proliferation of CTLL-2 cell, corresponding to a specific activity of > 1 x 10 5 IU/mg. Recommend Usage: Biological Activity ... Store at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20°C to -80°C. ... WebCTLL-2 cells were seeded in a 96-well plate and treated with varying concentrations of Human Recombinant IL-2. Cell proliferation was measured using a colorimetric assay. In the graph below, the specific … WebAliquots can be stored between 2°C and 8°C for up to one week and stored at -20°C or colder for up to 3 months. Avoid repeated freeze/thaw cycles. Activity ED 50 = 0.3 - 1.8 ng/ml, corresponding to a specific activity of 0.55 - 3.3 x 10 6 units/mg, as determined by CTLL-2 cell proliferation induced by mouse IL-4 in a dose dependent manner. how it\u0027s made wigs

LEADGMP ® IL-2 (Interleukin-2), Human - Leadgene Biomedical

Human CD25 (IL-2R alpha) Recombinant Protein (34-8702 …

WebIL-15 was discovered in the supernatant from a simian kidney epithelial cell line CV-1/EBNA, as a soluble factor capable of supporting proliferation of the IL-2-dependent cell line, CTLL-2 (1). Interleukin-15 (IL-15) is a regulatory cytokine, and it is produced by dendritic cells, epithelial cells, human stromal cell line (IMTLH), fibroblasts ... Web1.875 x 10^4. HT-2. 2.151 x 10^4. The specific activity (units/mg) of a cytokine is typically calculated using a bioassay with one set of conditions and parameters, and a defined cell line. Due to inherent variability, the specific activity measured with a different cell line, cultivated under different conditions in a different lab, can differ ... how i turned into a slime animeWeb一种重组人il2包涵体纯化复性方法技术领域 1.本技术涉及一种重组人il2包涵体纯化复性方法，属于蛋白表达纯化技术领域。背景技术： 2.白细胞介素2(il2)又称t细胞生长因子，是由t细胞(cd4+和cd8+)和nk细胞产生，主要受抗原和t细胞受体结合刺激分泌。 il2基因位于染色体4q26-28,长度3662bp,含4个外显子和3 ... how i turned into a slime

"WebEach lot has its own ED50 determined using an activity assay based on a specific function of the growth factor. The activity of our IL2 CTS™ Recombinant Human growth factor is … " - Ctll-2 ed50

Ctll-2 ed50

Human CD25 (IL-2R alpha) Recombinant Protein (34-8702 …

Webctll-2 Cells. Organism Type. Mouse. Cytotoxic T cell line (CD8+) Subculturing: Subculture actively growing suspension cultures before they the reached 1 X 10 exp5 cell/ml. Use … WebED50 : 0.5 ng/mL; determined by the dose-dependen proliferation of mouse CTLL-2 cells. Conjugate: Unconjugated : Form: Lyophilized: Purification: Size-exclusion - Gel filtration: Contains: ... Interleukin 2 (IL-2) is an immuno-modulatory cytokine that is important for the proliferation of activated T cells, differentiation of B cells, natural ...

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WebThe present invention relates to cancer immunotherapy. In particular, provided herein are fusion proteins for targeting tumor associated macrophages with immunostimulatory agents. WebMeasure by its ability to induce proliferation in CTLL-2 cells. The ED50 for this effect is <0.2 ng/mL. The specific activity of recombinant human IL-2 is approximately >2.5 x 107 IU/mg. To demonstrate lot-to-lot consistency of GMP IL-2, the activity of three separate lots was examined and plotted on the same graph.

WebIL-2 stimulates growth and differentiation of B-cells, NK cells, lymphokine activated killer cells, monocytes, macrophages and oligodendrocytes. ... Determined its its ability to … WebR&D Systems™ Recombinant Human IL-2 Protein. Version with mutation found in aldesleukin Available! Supplier: R&D Systems™ 202IL050. View more versions of this …

WebIL2, IL-2, TCGF, lymphokine. Bioactivity. The ED50 as determined by the dose-dependent stimulation of murine CTLL-2 cells is ≤ 0.1 ng/ml, corresponding to a specific activity of ≥ 1 x 107 units/mg. Purification Method. SDS-PAGE & … WebInterleukin-2 (IL-2), originally called T cell growth factor (TCGF), is a multifunctional cytokine which can stimulate the differentiation and proliferation of T lymphocytes and other cell types including B lymphocytes, NK cells, LAK cells, and monocytes/macrophages. ... ED50 = 10 - 150 pg/mL, has previously been reported using CTLL-2 as ...

WebCTLL-2 cell proliferation: ED50 ≤ 5 ng/mL: Preparation and Storage Country of Origin: USA Shipping: Room temperature Storage Prior to Reconstitution: -20°C Product Reconstitution: Sterile 10 mM sodium bicarbonate, pH 8.5 at 0.1 mg/mL Instructions: Centrifuge vial before opening. Suspend the product by gently pipetting the above recommended ...

WebJun 2, 2012 · FIGURE 61.1. A bioassay for IL-2 using CTLL-2 cells. Supernatants of tumor cells (PCl-13) transduced with the gene for human IL-2 were collected and tested for the … how i turned my husband into a womanWebBioactivity: The ED50 of this protein, as measured by CTLL-2 cell proliferation assay, is less than or equal to 4 ng/mL. This corresponds to a specific activity of greater than or equal to 2.5 x 10e5 Units/mg. Endotoxin: Less than 0.1 ng/ug cytokine as determined by the LAL assay. Purity: >98% as determined by SDS-PAGE. Molecular Weight: 29.4 kDa. how i turned my hobby into a business how i turned my husband biWebMeasured in a cell proliferation assay using CTLL‑2 mouse cytotoxic T cells. Gearing, A.J.H. and C.B. Bird (1987) in Lymphokines and Interferons, A Practical Approach. Clemens, M.J. et al. (eds): IRL Press. 295. The ED50 for this effect is 0.0300-0.250 ng/mL. Source. E. coli-derived human IL-2 protein Ala21-Thr153 (Cys145Ser), with and ... how i turn off my computerWebThe ED50 as determined by the dose-dependent stimulation of the proliferation of murine CTLL-2 cells is ≤ 0.4 ng/ml, corresponding to a specific activity of ≥ 2.5 x 10^6 units/mg. ... natural killer cells, monocytes and macrophages. IL-2 signals through the IL-2 receptor (IL-2R), a heterotrimeric protein complex whose gamma chain is also ... how i turned to be optimisticWebBackground: Interleukin 2 (IL-2) is a T cell stimulatory cytokine best known for inducing activated T cell proliferation . in vitro ... Bioactivity: ED50 is determined by the dose-dependent Proliferation of CTLL- 2 cells. The ED50 is typically less than 3 ng/mL. The international units of CTGrade rh IL-2. how i turned to scienceWebMeasure by its ability to induce proliferation in CTLL-2 cells. The ED 50 for this effect is 3 ng/mL. ... (Interleukin-2) protein, Human (60701GMP) stimulates the proliferation of CTLL-2 cells. The ED50 is 1.5 ng/mL. IL2 (Interleukin-2) protein, Human (60701GMP) was separated on SDS-PAGE under the reducing condition and visualized by Coomassie ... how i turned my life around