WebBenzo(a)pyrene (BaP), a polycyclic aromatic hydrocarbon, is considered a common endocrine disrupting chemical (EDC) with mutagenic and carcinogenic effects. In this work, we evaluated the effects of BaP on the hypothalamo-pituitary-gonadal axis (HPG) of zebrafish embryos. The embryos were treated with 5 and 50 nM BaP from 2.5 to 72 … WebThe DHFR-gfp was used to construct a small circular chromosome and to target gene disruption in the parasite. For construction of the small circular chromosome (DHFR-gfp …

OECD Work Related to Endocrine Disrupters - OECD

WebJul 24, 2024 · The ultimate blocking of autophagic flux by tetrandrine led to the disruption of mitochondria remodeling and inhibition of myogenic differentiation. The inhibitory effects of tetrandrine on skeletal muscle differentiation may limit … WebAssays, Gene delivery, Genetics, Imaging probes, Polyethylenimine Abstract Carbon dots (CDs) have become the focus of many studies due to their outstanding optical properties and good biocompatibility. We investigated their potential application to produce a smart and highly efficient yet nontoxic nanovector for gene delivery. mitch reznick federated hermes

Stable expression of green fluorescent protein and targeted …

WebOverview of mitochondria function assays. The disruption of mitochondria function can be detected using a variety of fluorescence-based assays including measurements of … GFP fusion proteins are made by connecting its DNA code to the cDNA encoding for another protein. Effectively, it equips the protein of interest with an additional protein module that can report on location. This approach enables the study of proteins in cells, but not other biomolecules of interest e.g. DNA, … See more Chromophore formation requires a fully folded beta-barrel structure, followed by an intramolecular reaction that generates the chromophore (Tsien, … See more The chromophore of GFP can exist in a protonated and a deprotonated state (Tsien, 1998). The deprotonated state has an absorbance maximum around 488 nm and it emits light that … See more The cyan light that is used to excite GFP, may also excite several components that are naturally present in cells or media. These sources of ‘autofluorescence’ include riboflavin in … See more GFP is a 28 kDa protein that resembles a cylinder with a length of 4.2 nm and a diameter of about 2.4 nm (Hink et al., 2000). The complete … See more WebVisualization and targeted disruption of protein interactions in living cells Protein-protein interactions are the basis of all processes in living cells, but most studies of these interactions rely on biochemical in vitro assays. mitch rhoades