Hieff clone plus multi one step cloning kit
Web9 de dez. de 2024 · Then, the upstream (before the start codon of the gene) and downstream (after the stop codon of the gene) fragment of each gene and the enzymatically digested vector were mixed together by seamless cloning with the Hieff Clone® Plus Multi One Step Cloning Kit (YEASEN, Shanghai, China), to get the plasmids pKC1139S … WebDescription. This kit is further developed based on the principle of efficient and rapid connection of DNA fragments by topoisomerase. Compared with the traditional T4 ligase, …
Hieff clone plus multi one step cloning kit
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WebThe Hieff Clone™ Universal One Step Cloning Kit should be stored at -25℃~ -15°C for 1 year. Figures. Figure 1 Multi-segment linkage: performance comparison with competitive … Web14 de fev. de 2024 · 图8：Hieff Clone TM Plus Multi One Step Cloning Kit与不同品牌的同类产品相比，显示出更高的性价比。 FAQ. Q1 ： Hieff Clone TM 克隆的原理是什 …
Web11 de nov. de 2024 · The In-Fusion cloning based on in vitro homologous recombination was performed to generate all the constructs using an In-Fusion HD Cloning Kit (Clontech) and Hieff Clone Plus Multi One Step ... Web27 de abr. de 2024 · PCR of the coding sequence of Met or Gce from the pClone007-Met or pClone007-Gce vector was performed, and then we used a Hieff Clone Plus Multi One Step Cloning Kit (10912ES10, Yeasen) for assembly. Finally, we obtained the pUAST-Met-4 × FLAG-Avi-P2A-BirA ( UAS-Met-BirA ) or pUAST-Gce-3 × FLAG-Avi-P2A-BirA ( UAS …
WebHieff Clone ® Universal One Step Cloning Kit 是新一代的同源重组克隆试剂盒，精心优化的 2×Hieff Clone ® Universal Enzyme Premix 预混了重组酶和重组反应所需缓冲液，并添加了独特的重组增强因子，可显著提高重 … Web1 de jul. de 2024 · Secondly, fragments of EGFP coding sequence (amplified with EGFP-ORF-F and EGFP-ORF-R) and Pxvasa 3′UTR (amplified with vasa3′UTR-F and vasa3′UTR-R) were used to construct two transient expression vectors vasaP2k-EGFP-SV40 and vasaP1214-EGFP-vasa3′UTR using Hieff Clone Plus Multi One Step Cloning Kit …
Web12 de ago. de 2024 · The full-length cDNA of OsEBF1 (LOC_Os06g40360) was amplified with KOD-plus DNA polymerase (Toyobo, Tokyo, Japan) using the primers EBF1OE-attB-F and EBF1OE-attB-R, and cloned into the p1301-35SNos vector using a Hieff-Clone™ Plus Multi One Step Cloning Kit (Yeasen, Beijing, China).
WebVazyme ClonExpress II One Step Cloning is a simple, fast, and high efficient cloning technology and enables directional insertion of any amplified DNA product into any linearized vector at any site. Firstly, the vector is linearized at the cloning site. A small sequence overlapped with each end of the cloning site is added onto the insert ... theoretical estimateWeb20 de mai. de 2024 · The purified PCR product was then cloned into the pCAMBIA1300-UBI-RBCS vector using the Hieff Clone® Plus One Step Cloning Kit. The D3 knockout vector was constructed using the CRISPR/Cas9 method. Based on the genomic sequence of D3 gene, two specific CRISPR target sites were designed in the protein-encodable … theoretical evaluation of overflow techniquesWebUniversal one step cloning kit, a one step cloning kit, is developed by seamless cloning technology based on homologous recombinase. Compared with the traditional enzyme ligation technology, the seamless cloning kit is simple and fast to operate, and is not limited by the restriction of the enzyme cleavage site. It can splicing multiple fragments at one … theoretical etymologyhttp://www.bioon.com.cn/news/showarticle.asp?newsid=68004 theoretical ethical modelhttp://yeasen.com/assets/upload/file/20240817130924833.pdf theoretical ethicsWebGenBuilder™ Cloning Kit: GenBuilder™ Plus Cloning Kit: 1: Unique need fulfilled by the kit: Multi-gene cloning for up to 6 fragments: Multi gene cloning for up to 12 fragments: 2: Cost per reaction: $7.90: $9.80: 3: Number of fragments that can be cloned into a linearized vector: 6: 12: 4: Cloning efficiency (insert was cloned into the ... theoretical evaluation definitionWebThe GenBuilder Plus Cloning Kit enables seamless and one-step cloning of multiple DNA fragments into a linearized vector. The proprietary enzyme mix fuses together DNA fragments efficiently and precisely by recognizing 15-40 bp overlaps at the end of each fragment. The system allows cloning of DNA fragments into any theoretical ethical framework