WebThe N2a cells were transiently transfected with the flag-CaMKIIα and Pin1 (WT, W34A, R68/69A) plasmids. After 24–48 h, the cells were lysed in the extraction buffer. The cell lysates were analyzed using a CaMKII Assay Kit (Promega). The substrate peptide of this kit contains no Ser/Thr-Pro site to which Pin1 can bind. WebPIN1. The human parvulin PIN1 (EC 5.2.1.8, 163aa) is a nuclear protein implicated in the regulation of mitosis through interaction with CDC25 and polo-like kinase-1. From: The …
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WebApr 9, 2024 · Assay Development. Immunohistochemistry; Antibody Pair; Proteoscreen® Tissue Profiling; Companion Diagnostics; FISH Probes; mutaFISH™ Probes; ELISA Kits; Neutralizing Antibody Assay; Tumor Animal Models. Orthotopic Xenograft Tumor Models; Patient-Derived Xenograft Tumor Models; Circulating Tumor DNA. Circulating Tumor … WebPIN1 ELISA Kits. The ELISA kits listed below target PIN1, the symbol for the human gene, peptidylprolyl cis/trans isomerase, NIMA-interacting 1. The protein has an enzyme …
WebFeb 23, 2024 · The cell viability in each group was evaluated using a CCK8 assay kit (MCE, NJ, USA). The optical density values at a wavelength of 450 nm were measured using a microplate reader to determine cell ... WebSep 2, 2024 · (D-F) ATRA, ATO, Pin1i-1 and Pin1i-2 dose-dependently suppress cell growth and ablate Pin1. PDAC cells were treated with ATRA, ATO, Pin1i-1 or Pin1i-2 for 72 hr, followed by examining cell growth using proliferation assay (D, F) and Pin1 using IB (E). ATRA and ATO were used in 10: 1 ratio as Pin1i-1 and only ATRA concentrations are …
WebPin1 is a PPIase specific for pSer/pThr-Pro motifs; Uses recombinant human Pin 1 and substrate Succ-AEPF-pNA; Measures cis/trans isomerisation kinetics of substrate using … Web17-313. Sigma-Aldrich. PP2A Immunoprecipitation Phosphatase Assay Kit. PP2A Immunoprecipitation Phosphatase Assay Kit can be used to detect purified PP2A or PP2A from a cellular lysate. It utilizes the Malachite Green & contains components that have been matched to o. MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, …
WebPin1 can bind phosphorylated Thr-212/231 residues of Tau protein and increase its deposphorylation that may prevent formation of Tau neurofibrilary tangles. The …
WebSensoLyte® 520 HDAC Activity Assay Kit Fluorimetric. Order Now. HAT (Histone acetyltransferase) SensoLyte® HAT (p300) Assay Kit Fluorimetric. Order Now. HCV Protease. SensoLyte® 490 HCV Protease Assay Kit Fluorimetric 500 assays. Order Now. SensoLyte® 490 HCV Protease Assay Kit Fluorimetric 200 assays. ghisa power 18 x xm-l t6WebOct 5, 2024 · For Pin1 knockdown, lentivirus LV3-Pin1, pKLO plasmid and inhibitors ATRA and Juglone were used following the manufacturer’s instructions. 2.3. Western blot. Briefly, tissue and cell lysates were extracted with RIPA buffer. Protein concentration was measured by BCA Protein Assay Kit. ghisa termosifoniWeblung tissue (lysates) were assessed using ELISA kits from R&D Systems. The sensitivity of detection was 7 pg/ml. GST Pull-down Assay—GST-Pin1 and His-Smad6 (cDNA from Addgene) were expressed in bacteria, and the pull-down assay was performed as described previously (13). Immunostaining and Confocal Analysis—For immunofluo- ghisa in tedescoWebAnaSpec (Eurogentec's US subsidiary) was pioneer in developing the first ELISA kits for monitoring the presence of anti-MOG and anti-PLP autoantibodies in body fluids. Our MOG kits are flexible, as they can detecting autoimmune IgGs against mouse/rat MOG (35-55), rat MOG (1-125), mouse MOG (1-125) or human MOG (1-125). chromatin appearanceWebRayBio® Human Pin1 ELISA Kit for Serum, Plasma, and Cell Culture Supernatants.Lead time: Typically ships within 1-2 business days. No Friday shipments. ghis basic price listWebView（）/((6E,8E,10E,12E,14E,16E,18E,20E,22E,24E,26E)-2,6,10,14,19,23,27,31-Octamethyldotriaconta-2,6,8,10,12,14,16,18,20,22,24,26,30-tridecaene) information and ... chromatin assembly assayWebThe SensoLyte® Green Pin1 Assay Kit uses a fluorogenic substrate pretreated to convert into cis isoform. Pin1 changes this substrate into the trans conformation that is readily … chromatin are made up of