Small highly active dnas that hydrolyze dna

Author: jdws

August undefined, 2024

WebbThe development of a simple DNA sensor based on DNAzyme and PCR and aimed at the detection of low concentrations of zinc (II) ions, which showed sensitivity (10 nM) and high specificity for zinc ion detection is described. AbstractThe development of a simple sensor (9NL27-Zn) based on DNAzyme and PCR and aimed at the detection of low …

DNAmoreDB - A Database of Deoxyribozymes

WebbNatural DNA sequences conforming to the class I consensus sequence and structure were found that undergo hydrolysis under selection conditions (2 mM Zn2+, pH 7), which … Webb6 juni 2013 · Natural DNA sequences conforming to the class I consensus sequence and structure were found that undergo hydrolysis under selection conditions (2 mM Zn(2+), … rca with ground

Engineering a DNA-cleaving DNAzyme and PCR into a simple …

WebbNucleotides in black are present in the parent construct used for reselection, but these positions are not well conserved in the selected population. Arrows designate the site of … WebbSmall, highly active DNAs that hydrolyze DNA. Article. Full-text available. May 2013; J AM CHEM SOC; Hongzhou Gu; Kazuhiro Furukawa; Zasha Weinberg; Daniel F Berenson; Ronald R Breaker; Webb(B) Hydrolytic instability of natural DNA sequences. Trace amounts of each natural DNA (5′ 32P-labeled) were incubated at 37 °C in reaction buffer without or with 2 mM Zn2+ for 6 … sims 4 maternity shoot clothes

Small, Highly Active DNAs That Hydrolyze DNA Journal of the American

Production of single-stranded DNAs by self-cleavage of rolling …

Webb14 okt. 2024 · Catalytic DNAs, mainly DNA/RNA-cleaving DNAs, ... with the total 17 conserved nucleotides forming a catalytic loop to site-specifically hydrolyze DNA in the loop upon the supplementation of Zn 2+ ... Small, highly active DNAs that hydrolyze DNA. J. Am. Chem. Soc., 135 (2013), pp. 9121-9129. WebbLigation of linear single-stranded DNAs with CircLigase to form circles. CircLigase is a thermostable ATP-dependent single-stranded DNA ligase that catalyzes intramolecular ligation of single-stranded DNA templates. The ligation substrates must carry 5′-phosphate a 3′-hydroxyl groups on the donor and acceptor DNAs, respectively. sims 4 maternity photoshoot ccWebbhydrolyzing DNAs is further highlighted by the fact that it has been relatively straightforward to create deoxyribozymes that promote DNA cleavage by oxidative 4 or … rcaw trade show

"WebbMembers of the single-stranded DNA pool contain two 15 nt primer binding sites (5′ and 3′ termini) and two 50 nt random-sequence domains ... Reported in... Year of Publication First Author Laboratory Title PubMed ID DOI Reaction; 2013: H Gu: R R Breaker: Small, highly active DNAs that hydrolyze DNA. 23679108: 10.1021/ja403585e: DNA cleavage ... " - Small highly active dnas that hydrolyze dna

Small highly active dnas that hydrolyze dna

Merely two mutations switch a DNA-hydrolyzing deoxyribozyme …

WebbNatural DNA sequences conforming to the class I consensus sequence and structure were found that undergo hydrolysis under selection conditions (2 mM Zn2+, pH 7), which … WebbDNA, Catalytic "DNA, Catalytic" is a descriptor in the National Library of Medicine's controlled vocabulary thesaurus, MeSH (Medical Subject Headings). Descriptors are arranged in a hierarchical structure, which enables …

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WebbNatural DNA sequences conforming to the class I consensus sequence and structure were found that undergo hydrolysis under selection conditions (2 mM Zn 2+, pH 7), which … Webb6 okt. 2024 · Flow cytometry analysis. The MCF-7 cells (human breast tumor cell line), HeLa cells (cervical tumor cell line), and MCF-10A cells (human breast normal cell line) were seeded at a density of 2 × 10 5 per well onto a 6-well plate and cultured. When the cells reached 90% confluency, the F-ZD nanoparticles dispersed in the Opti-minimum …

Webb27 jan. 2011 · Small, highly active DNAs that hydrolyze DNA. Hongzhou Gu, K. Furukawa, Z. Weinberg, Daniel F. Berenson, R. Breaker; Biology. ... Using in vitro selection, this work has identified deoxyribozymes that sequence-specifically hydrolyze DNA with multiple turnover and rate enhancement of 108 ... Webb6 okt. 2010 · Small, Highly Active DNAs That Hydrolyze DNA. Journal of the American Chemical Society 2013, 135 (24) , 9121-9129. DOI: 10.1021/ja403585e. Darren J. Parker, Ying Xiao, John M. Aguilar, and Scott K. Silverman . DNA Catalysis of a Normally Disfavored RNA Hydrolysis ...

WebbMembers of the single-stranded DNA pool contain two 15 nt primer binding sites (5′ and 3′ termini) and two 50 nt random-sequence domains ... Reported in... Year of Publication … Webbcorresponding / co-corresponding author * co-first author I. Eckert, R. Friedrich, C.E. Weinberg, Z. Weinberg (2024) Discovery of natural non-circular permutations in non-coding RNAs, Nucleic Acids Res, online ahead of print. F. Lenkeit, I. Eckert, M. Sinn, F. Hauth, J.S. Hartig, Z. Weinberg (2024) A variant of guanidine-IV riboswitches exhibits evidence of a …

WebbDNA cleavage by 10MD5 proceeds with kobs = 2.7 h −1 and rate enhancement of 10 12 over the uncatalyzed P−O hydrolysis reaction. 10MD5 has a very sharp pH optimum near 7.5, with greatly reduced DNA cleavage rate and yield when the pH is changed by only 0.1 unit in either direction.

Webbför 2 dagar sedan · Herein, based on the mobility change of DNA in denaturing gels, we develop a selection strategy to discover a series of 40-45 nt small DNAs that can bind … sims 4 maternity suite buildWebb5 juni 2013 · Natural DNA sequences conforming to the class I consensus sequence and structure were found that undergo hydrolysis under selection conditions (2 mM Zn(2+), … rca wsp150 transmitterWebb6 juni 2013 · The identification of small DNA-cleaving deoxyribozymes that function with high speed under mild reaction conditions should permit their use in various … sims 4 maternity shootWebb16 maj 2013 · highly-active deoxyribozymes that catalyze DNA hydrolysis indeed can be isolated. However, the precise sequence at the cleavage site and its structural … rca wsp200 wireless speakers manualWebb1 juni 2013 · I-R3 DNAzyme is a small, highly active catalytic DNA for DNA hydrolysis. In here, we designed two cis-structure DNAzymes (I-R3N and I-R3S) based on the different locates of the joint linker ... rcb16f200cWebb6 juni 2013 · DNA phosphoester bonds are exceedingly resistant to hydrolysis in the absence of chemical or enzymatic catalysts. This property is particularly important for … sims 4 maternity photoshoot clothesWebb7 dec. 2024 · DNA nanotechnology, in particular DNA origami, enables the bottom-up self-assembly of micrometre-scale, three-dimensional structures with nanometre-precise features 1,2,3,4,5,6,7,8,9,10,11,12 ... sims 4 maternity photoshoot